XP725

Sigma

 

Panorama® Antibody Microarray - XPRESS Profiler725 Kit

Description

ApplicationThe array can be used for comparing protein expression profile between two samples (test vs. reference samples). Each sample is labeled with a different dye (Cy3 or Cy5) and the 2 samples are applied simultaneously on the array. The expression profile of each sample (Cy3 and Cy5) is recorded individually and compared.
General descriptionThe Panorama Antibody Microarray - XPRESS Profiler725 Kit is a proteomics tool that measures the relative level of protein expression (protein microarrays) in cell or tissue extracts. It contains 725 antibodies on nitrocellulose coated glass slides.
LinkageLearn more about the XPRESS Profiler725 array at www.sigma.com/xp725. For expression studies involving custom peptide libraries, visit www.sigma-aldrich.com/pepscreen.
Other NotesPlease note that featured antibody content is lot specific and is subject to change.
 To view Panorama® array literature, visit www.sigma-aldrich.com/arrayliterature
Legal InformationBenzonase® Nuclease is manufactured by Merck KGaA and its affiliates. The technology is covered by US Patent 5,173,418 and EP Patent 0,229,866. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase® Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany. Benzonase® is a registered trademark of Merck KGaA, Darmstadt, Germany.
Cy is a trademark of Amersham Pharmacia Biotech, UK Ltd.
quadriPERM is a registered trademark of Heraeus Instrument GmbH.
TWEEN is a registered trademark of Uniqema, a business unit of ICI Americas Inc.
Features and Benefits• Accurate & robust protein expression profiling against 725 different antibodies in half a day.
• Suitable for use with Cy3/Cy5 -based detection chemistries.
• Proprietary slide treatment minimizes background staining.
ยท
 Panorama is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

shipped inwet ice
storage temp.2-8°C

Safety

Hazard CodesC
Risk Statements34
Safety Statements26-36/37/39-45
RIDADRUN 3316 9/PG 2

Components

Kit component onlyPanorama Antibody Slides - XPRESS Profiler725 2
 quadriPERM® Cell Culture Vessels 2
 Extraction/Labeling Buffer 30 mL
 Protease Inhibitor Cocktail
 Phosphatase Inhibitor Cocktail II
 Benzonase®, Ultrapure
 Array Incubation Buffer 20 mL
 Phosphate buffered saline, pH 7.4, contains Tween 20, dry powder 1 ea
 SigmaSpin Post-Reaction Clean-Up Columns 8 ea
 SigmaSpin Post-Reaction Clean-Up Columns 16 ea
 Panorama Antibody List - XPRESS Profiler725 1 ea

Related Products

Related productG6171, Monoclonal Anti-Glial Fibrillary Acidic Protein antibody produced in mouse

References

referenceAndersson O, et al., Determination of relative protein abundance by internally normalized ratio algorithm with antibody arrays J. Proteome Res. 4, 758-767, (2005) Abstract
 Chaga GS., Antibody arrays for determination of relative protein abundances Methods Mol. Biol. 441, 129-151, (2008) Abstract
 Korf U, et al., Antibody microarrays as an experimental platform for the analysis of signal transduction networks Adv. Biochem. Eng. Biotechnol. 110, 153-175, (2008) Abstract
 Simara P, et al., Combination of mRNA and protein microarray analysis in complex cell profiling Neoplasma 56, 141-149, (2009) Abstract